imbb logo for mobile

Human Brain Organoids Facility (HBOF)

Brain organoids are 3D self-organising, complex structures of different regions of the brain, which are used as in vitro models to study brain development and brain disorders. We have established the protocols (Gkogkas lab) to generate forebrain (dorsal and ventral) and midbrain organoids from human pluripotent stem cells (induced; iPSC or embryonic; ESC).

Dorsal Forebrain Organoids
enriched in Excitatory neurons
Ventral Forebrain Organoids
enriched in Inhibitory neurons
Midbrain Organoids
enriched in dopaminergic neurons
Generation of human brain organoids from pluripotent stem cells


Projects that the HBOF undertakes:

  •     Precision Medicine Applications
  •     Basic/Pre-clinical Research
  •     Compound Screening
  •     Novel Therapeutics
  •     Toxicology
  •     Biomarker Discovery
  •     Diagnostics Applications

This facility includes: 2 incubators dedicated for work with brain organoids, a biosafety flow cabinet and and an EVOS™ XL Core Imaging System for visualization and imaging. The facility also includes bioreactors for large-scale preparations (>1000 brain organoids), automated cell counting (Countess 3 Automated Cell Counter) and gene-expression analysis with RT-qPCR (ARIA-Mx, Agilent).


Dr. Christos G. Gkogkas Tel: +30 26510 07363
Dr. Kleanthi Chalkiadaki Human Brain Organoids Facility, FORTH-BRI E5 Building, University Campus Ioannina
  Ioannina, 45110, GREECE


Regulation of mRNA translation in stem cells; links to brain disorders.
Statoulla E, Chalkiadaki K, Karozis D, Gkogkas CG.Cell Signal. 2021 Dec;88:110166.
doi: 10.1016/j.cellsig.2021.110166.

Translational control in neurovascular brain development.
Chalkiadaki K, Statoulla E & Gkogkas CG.R Soc Open Sci. 2021 Oct 13;8(10):211088.
doi: 10.1098/rsos.211088. eCollection 2021 Oct.