Πέτρος Μαραγκός
Η ομάδα μας ασχολείται με τη μελέτη της ρύθμισης του κυτταρικού κύκλου και την απόκριση σε βλάβες του DNA κατά τη διάρκεια της ανάπτυξης του ωοκυττάρου και του πρώιμου εμβρύου στα θηλαστικά.
Η ρύθμιση του κυτταρικού κύκλου κατά τη διάρκεια της γαμετογένεσης και της πρώιμης εμβρυικής ανάπτυξης παίζει σημαντικό ρόλο στην γονιμότητα και την ανάπτυξη του εμβρύου. Επιπλέον, οι δράσεις μειωτικών και βλαστοκυτταρικών ρυθμιστών εμπλέκονται στον έλεγχο της ογκογένεσης.
Ερευνητικές δραστηριότητες:
• Μελέτη του κυτταρικού κύκλου ωοκυττάρων, ωαρίων και προ-εμφυτευτικών εμβρύων θηλαστικών.
• Μελέτη της απόκρισης ωοκυττάρων, ωαρίων και προ-εμφυτευτικών εμβρύων σε βλάβες του DNA.
• Μελέτη της απόκριση εμβρυικών βλαστοκυττάρων σε βλάβες του DNA.
• Προσδιορισμός του ρόλου και της λειτουργίας ωοκυτταρικών ρυθμιστών που παρουσιάζουν και ογκογονική έκφραση.
• Μελέτη των μηχανισμών της γήρανσης ωοκυττάρων και της σχέσης της με χρωμοσωμικές ανωμαλίες, ανευπλοειδία και βλάβες του DNA.
• Μελέτη του κυτταρικού κύκλου των βλαστοκυττάρων της Νευρικής Ακρολοφίας.
Gonzalez Malagon SG, Dobson L, Muñoz AML, Dawson M, Barrell W, Marangos P, Krause M, Liu KJ. (2019) Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration. J Vis Exp. (152):10.3791/60051.
Nabti I, Grimes R, Sarna H, Marangos P, Carroll J. (2017) Maternal age-dependent APC/C-mediated decrease in securin causes premature sister chromatid separation in meiosis II. Nat Commun. 8:15346.
Marangos P, Stevense M, Niaka K, Lagoudaki M, Nabti I, Jessberger R, Carroll J. (2015) DNA damage-induced metaphase I arrest is mediated by the Spindle Assembly Checkpoint and maternal age. Nat Commun. 6:8706.
Marangos P, Carroll J. (2012) Oocytes progress beyond prophase in the presence of DNA damage. Curr Biol. 22:989-94.
Marangos P, Carroll J. (2008) Securin regulates entry into M-phase by modulating the stability of cyclin B. Nat Cell Biol. 10:445-51.
Rémillard-Labrosse, G., Dean, N. L., Allais, A., Mihajlović, A. I., Jin, S. G., Son, W. Y., Chung, J. T., Pansera, M., Henderson, S., Mahfoudh, A., Steiner, N., Agapitou, K., Marangos, P., Buckett, W., Ligeti-Ruiter, J., & FitzHarris, G. Human oocytes harboring damaged DNA can complete meiosis I. Fertility and Sterility, 2020, 113(5), 1080–1089.e2.
Gonzalez Malagon, S. G., Dobson, L., Muñoz, A. M., Dawson, M., Barrell, W., Marangos, P., Krause, M., Liu, K. J. Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration. JoVE (J. Vis. Exp.) 2019, (152), e60051, doi:10.3791/60051.
Zhang QH, Yuen WS, Adhikari D, Flegg JA, FitzHarris G, Conti M, Sicinski P, Nabti I, Marangos P, Carroll J. Cyclin A2 modulates kinetochore-microtubule attachment in meiosis II. J Cell Biol. 2017 Aug 17. 216:3133-3143.
Nabti I, Grimes R, Sarna H, Marangos P, Carroll J. Maternal age-dependent APC/C-mediated decrease in securin causes premature sister chromatid separation in meiosis II. Nat Commun. 2017 May 18;8:15346.
Marangos P. Preparation of cell lysate from mouse oocytes for Western blotting analysis. Methods in Molecular Biology. 2016;1457:209-15
Marangos P, Stevense M, Niaka K, Lagoudaki M, Nabti I, Jessberger R and Carroll J. DNA damage-induced metaphase I arrest is mediated by the Spindle Assembly Checkpoint and maternal age. Nature Communications. 2015 November 2;6:8706.
Ibtissem Nabti, Marangos P, Kudo N and Carroll J. Dual-mode regulation by CDK1 and MAPK controls APC activity during meiosis I in mouse oocytes. Journal of Cell Biology. 2014 March; 204(6):891-900.
Carroll J and Marangos P. The DNA damage response in mammalian oocytes. Frontiers in Genetics (Cancer Genetics). 2013 June 24;4:117.
Marangos P. Micro-injection of Morpholino oligonucleotides for depleting Securin in mouse oocytes. Methods in Molecular Biology. 2013; 957: 153-62
Marangos P and Carroll J. Oocytes progress beyond prophase in the presence of DNA damage. Current Biology, 2012 June; 22(11): 989-994.
Marangos P and Carroll J. Securin regulates entry into M-phase by modulating the stability of cyclin B. Nature Cell Biology, 2008 April; 10(4): 445-451.
Marangos P, Verschuren EW, Chen R, Jackson PK and Carroll J. Prophase I arrest and progression to metaphase I in mouse oocytes are controlled by Emi1-dependent regulation of APC/Cdh1. Journal of Cell Biology. 2007 Jan 1;176(1):65-75.
FitzHarris G, Marangos P and Carroll J. Changes in endoplasmic reticulum structure during mouse oocyte maturation are controlled by the cytoskeleton and cytoplasmic dynein. Developmental Biology. 2007 May 1;305(1):133-44.
Marangos P, Carroll J. Fertilization and InsP3-induced Ca2+ release stimulate a persistent increase in the rate of degradation of cyclin B1 specifically in mature mouse oocytes. Developmental Biology. 2004 Aug 1;272(1):26-38.
Marangos P, Carroll J. The dynamics of cyclin B1 distribution during meiosis I in mouse oocytes. Reproduction. 2004 Aug;128(2):153-62.
Dumollard R, Marangos P, Swann K, Duchen M and Carroll J. Sperm-triggered Ca2+ oscillations and Ca2+ homeostasis in the mouse egg have an absolute requirement for mitochondrial ATP production. Development. 2004 Jul;131(13):3057-67.
Carroll J, FitzHarris G, Marangos P, Hallet G. Ca2+ signalling and cortical re-organisation during the transition from meiosis to mitosis in mammalian oocytes. Eur J Obstet Gynecol Reprod Biol. 2004 Jul 1;115 Suppl 1:S61-7.
Marangos P. FitzHarris G. and Carroll J Ca2+ oscillations at fertilization in mammals are regulated by the formation of pronuclei. Development. 2003 Apr 1; 130(7):1461-1472
Halet G, Marangos P, FitzHarris G, Carroll J. Ca2+ oscillations at fertilization in mammals. Biochem Soc Trans. 2003 Oct; 31(Pt 5):907-11
FitzHarris G, Marangos P and Carroll J. Cell Cycle-dependent regulation of structure of endoplasmic reticulum and inositol 1,4,5-trisphosphate-induced Ca2+ release in mouse oocytes and embryos. Molecular Biology of the Cell. 2003 Jan;14(1):288-301
Christian M, Marangos P, Mak I, White J and Brosens J. Interferon-gamma modulates prolactin and tissue factor expression in differentiating human endometrial stromal cells. Endocrinology. 2001 Jul;142(7):3142-51