Brain organoids are 3D self-organising, complex structures of different regions of the brain, which are used as in vitro models to study brain development and brain disorders. We have established the protocols (Gkogkas lab) to generate forebrain (dorsal and ventral) and midbrain organoids from human pluripotent stem cells (induced; iPSC or embryonic; ESC).
|Dorsal Forebrain Organoids
enriched in Excitatory neurons
|Ventral Forebrain Organoids
enriched in Inhibitory neurons
enriched in dopaminergic neurons
|Generation of human brain organoids from pluripotent stem cells|
|Projects that the HBOF undertakes:
This facility includes: 2 incubators dedicated for work with brain organoids, a biosafety flow cabinet and and an EVOS™ XL Core Imaging System for visualization and imaging. The facility also includes bioreactors for large-scale preparations (>1000 brain organoids), automated cell counting (Countess 3 Automated Cell Counter) and gene-expression analysis with RT-qPCR (ARIA-Mx, Agilent).
|Dr. Christos G. Gkogkas||Tel: +30 26510 07363|
|Dr. Kleanthi Chalkiadaki||Human Brain Organoids Facility, FORTH-BRI|
|www.gkogkaslab.com||E5 Building, University Campus Ioannina|
|Ioannina, 45110, GREECE|
Regulation of mRNA translation in stem cells; links to brain disorders.
Statoulla E, Chalkiadaki K, Karozis D, Gkogkas CG.Cell Signal. 2021 Dec;88:110166.
Translational control in neurovascular brain development.
Chalkiadaki K, Statoulla E & Gkogkas CG.R Soc Open Sci. 2021 Oct 13;8(10):211088.
doi: 10.1098/rsos.211088. eCollection 2021 Oct.