Panos Kouklis
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EDUCATION/TRAINING |
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INSTITUTION AND LOCATION |
DEGREE |
YEAR |
FIELD OF STUDY |
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University of Athens, Greece |
BS |
1985 |
Pharmacy |
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University of Heidelberg - Max-Plank Institut for Cell Biology in Ladenburg - Germany |
PhD |
1990 |
Cell Biology |
Positions and Employment
- Predoctoral student in Max- Planck Institute for Cell Biology, - Ladenburg, F. R. Germany, 1985 – 1986
- Ph. D. thesis in Max- Planck Institute for Cell Biology, - Ladenburg, F. R. Germany, 1987 - 1989
- Post-doctoral fellow in the Cell Biology Program of EMBL (European Molecular Biology Laboratory, 1990 – 1992
- Research Associate in the Howard Hughes Medical Institute, University of Chicago, in the group of Prof. E. Fuchs, 1992 – 1995
- Instructor in the Department of Molecular Genetics and Cell Biology, University of Chicago, in the group of Prof. E. Fuchs, 1995 – 1998
- Research Assistant Professor in the Department of Pharmacology, University of Illinois - Chicago, 1998 - 2003
- Assistant Professor in the Department of Biology, Medical School – University of Ioannina – Greece, 2003 -
Honors and Awards
- Parker B. Francis Fellow, USA (1999 – 2002)
- Marie Curie Fellow, Greece (2005 – 2008)
1. A potential role for the COOH-terminal domain in the lateral packing of type III intermediate filaments.
P. D. Kouklis, T. Papamarcaki, A. Merdes, and S. D. Georgatos. (1991) J. Cell Biol. 114:773-786.
2. The “lamin B- fold”. T. Papamarcaki, P. D. Kouklis, T. Kreis, and S. D. Georgatos. (1991) J. Biol. Chem. 266:21247-21251.
3. Involvement of the consensus sequence motif at coil 2b in the assembly and stability of vimentin filaments.
P. D. Kouklis, P. Traub, and S. D. Georgatos. (1992) J. Cell Sci. 102:31-41.
4. In vitro assembly properties of vimentin mutagenised at the beta-site tail motif. P. D. Kouklis, M. Hatzfeld, M. Brunkener, K. Weber, and S. D. Georgatos. (1993). J. Cell Sci. 106:919-928.
5. Transient arrest of 3T3 cells in mitosis and inhibition of nuclear lamina reassembly around chromatin induced by an anti- vimentin antibodies. P. D. Kouklis , A. Merdes, T. Papamarcaki, and S. D. Georgatos. (1993) Eur. J. Cell Biol. 62:224-236.
6. The roles of the rod end and the tail in vimentin IF assembly and IF network formation. M. B. McCormick, P. D. Kouklis, D. Syder, and E. Fuchs. (1993) J. Cell Biol. 122:395-408.
7. Making a connection: Direct binding between keratin intermediate filaments and desmosomal proteins. P. D. Kouklis, E. Hutton, and E. Fuchs. (1994) J. Cell Biol. 127:1049-1060.
8. A new type of cytoskeletal protein: Connecting actin microfilaments to intermediate filaments. Y. Yang, J. Dowling, Q.C. Yu, P. D. Kouklis, D. Cleveland, and E. Fuchs. (1996) Cell 86:655-665.
9. Desmoplakin is required early in development for stabilization of desmosomes and cytoskeletal linkage.
G. I. Gallicano*, P. Kouklis*, C. Bauer, M. Yin, V. Vasioukhin, L. Degenstein, and E. Fuchs. (1998) J. Cell Biol. 143:2009-2022 *first co-authors
10. Increased microvessel permeability and its reversibility in mouse lung in response to VE-cadherin disassembly and reassembly. X. Gao, P. Kouklis, N. Xu, R. D. Minshall, R. Sandoval, S. M. Vogel and A. B. Malik. (2000). Am J Physiol Lung Cell Mol Physiol. 279:L1218-L1225
11. Ca 2+ signalling and PKCa activate increased endothelial permeability by dissasembly of VE-cadherin junctions. (2001). R. Sandoval, A. B. Malik, R. D. Minshall, P. Kouklis, C. A. Ellis and C. Tiruppathi.
J. Physiol. 533:433-445.
12. VE-cadherin induced Cdc42 signaling regulates formation of membrane protrusions in endothelial cells. (2003) P. Kouklis, M. Konstantoulaki and A. B. Malik. J. Biol Chem. 278:16230-16236.
12. Protein Kinase C modifications of VE-cadherin, p120, and b-catenin contribute to endothelial barrier dysregulation induced by thrombin. (2003). M. Konstantoulaki, P. Kouklis and A. B. Malik Am J Physiol Lung Cell Mol Physiol. 285(2):L434-42.
13. Cdc42 involvement in restoration of endothelial junctional integrity (2004).P. Kouklis, M. Broman, S. Vogel and A. B. Malik. Circulation Research 94:159-166
14. Cdc42 regulates adherens junction stability and endothelial permeability by inducing alpha-catenin interaction with the vascular endothelial cadherin complex (2006). Broman, M. T., P. Kouklis, et al Circulation Research 98(1): 73-80.
15. Regulation of Lung Neutrophil Recruitment by VE-cadherin (2006). Orrington-Myers, J., X. P.Gao, P. Kouklis, M. Broman, A. Rahman, A., Vogel,S. and Malik, A. B. Am J Physiol Lung Cell Mol Physiol. 291(4):L764-L771
16. Ramchandran, R., Mehta, D., Vogel, S. M., Mirza, M. K., Kouklis, P. and Malik, A. B. (2008). Critical role of Cdc42 in mediating endothelial barrier protection in vivo. Am J Physiol Lung Cell Mol Physiol 295 (2): 363-369.
17. Nikitopoulou, I., Orfanos, SE., Kotanidou, A., Maltabe, V., Manitsopoulos, N., Karras, P., Kouklis, P., Armaganidis, A., Maniatis, NA. (2016) Vascular endothelial-cadherin downregulation as a feature of endothelial transdifferentiation in monocrotaline-induced pulmonary hypertension. Am J Physiol Lung Cell Mol Physiol. 311(2):L352-63.
18. Maltabe, VA., Barka, E., Kontonika, M., Florou, D., Kouvara-Pritsouli, M., Roumpi, M., Agathopoulos, S., Kolettis, TM., Kouklis, P. (2016) Isolation of an ES-Derived Cardiovascular Multipotent Cell Population Based on VE-Cadherin Promoter Activity. Stem Cells Int.;2016:8305624. doi: 10.1155/2016/8305624.